KMID : 0380420230470030158
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Journal of Prventive Veterinary Medicine 2023 Volume.47 No. 3 p.158 ~ p.164
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Unveiling the potent antioxidant potential of Tulsi (Ocimum sanctum) leaf and stem extract through multi-method screening
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Most Nusrat Zahan
Kim Jong-Hyuk Yang Hye-Ryeon Park Sin-Yeong Kim Min-Yeong Rho Il-Rae Kim Eui-Kyung Kang Chang-Keun
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Abstract
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Tulsi (Ocimum sanctum), commonly known as Holy Basil is a revered herb with a rich history in traditional medicine systems, particularly in Southeast Asia. For its medicinal properties, Tulsi has been regarded as an ¡°Elixir of Life¡± and has been used to treat various ailments. However, the comprehensive investigation of Tulsi extracts and their potential pharmacological benefits, specifically in relation to antioxidant activity remains limited. Hence, the objective of this study was to evaluate the antioxidant activity of Tulsi leaf and stem extract using various screening methods. We investigate the antioxidant activity exhibited by the extract using three different methods involved the utilization of the total polyphenol content assay, the ferric reducing power assay and 2, 2-diphenyl-1-14 picrylhydrazyl (DPPH) assays. The results revealed that the Tulsi leaf extract (TLE) exhibited significantly higher antioxidant activity when compared to the Tulsi stem extract (TSE) in all the performed assays. The higher content of phenolics in TLE may have contributed to its superior antioxidant activity. The HPLC (high performance liquid chromatography) analysis of TLE revealed the presence of eugenol, active compound for several therapeutic properties. These findings provide an understanding of the bioactive compounds present in Tulsi extracts and their potential antioxidant benefits.
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KEYWORD
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Ocimum sanctum, Tulsi, Polyphenol, Antioxidant activity, HPLC analysis
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